Access

The facility is open to:

  • Researchers at Lund University
  • National and international academic users
  • Industrial partners through collaboration or service agreements

Please contact us at: cryoem-facility@med.lu.se

Instrumentation

Thermo Fisher Glacios 2 Cryo-TEM (200 kV)

Image003
  • Equipped with a Falcon 4i direct electron detector for high-speed, high-quality imaging
  • Autoloader with 12-grid capacity for automated, high-throughput data collection
  • High-stability cryo-stage optimized for long acquisitions
  • Fully integrated with EPU software for automated SPA and tomography workflows
Accelerating voltageFlexible accelerating voltage from 80–200 kV
Electron sourceHigh-brightness field emission gun (X-FEG)
Lens systemAutomatic condenser, objective, and SA apertures

Symmetric constant power objective lens (minimize image aberrations and lens hysteresis during mode switching)

Symmetric constant power C-TWIN objective lens with wide-gap pole piece (11 mm)
DetectorCamera architectureDirect electron detection
Sensor size4,096 x 4,096 pixels, ~5.7 cm x 5.7 cm2
Pixel size14 x 14 μm2
TEM operating voltage200 kV
Internal frame rate320 fps
Frame rate to storage320 fps (EER mode)
File formatsEER (native), MRC, TIFF, LZW TIFF
AFIS (aberration-free image shift)Enhancing throughput with shorter relaxation times when moving coma-free between grid holes
FFI (fringe-free imaging)Fringe-free imaging (FFI) for multiple image acquisitions per grid hole
Smart EPU SoftwareEPU 3 Software for automated sample screening and data acquisition 

EPU Multigrid functionality 

EPU Quality Monitor Software 

Sample preparation laboratory

  • Thermo Fisher Vitrobot Mark IV for plunge-freezing and vitrification
  • Glow discharger
  • Grid clipping and transfer tools for safe handling of cryo-grids
Thermofisher Vitrobot MarkIV
Thermofisher Vitrobot MarkIV
EasiGlow
EasiGlow
Quorum GLOQUBE
Quorum GLOQUBE

Computational resources

Current and planned resources:

  • A local GPU-enabled workstation, Abisko and Lofoten for data processing during data collection
  • A 380 TB landing stage from which users can retrieve their data using their DUO account (coming soon)
  • The most popular cryo-EM software is installed on Lund University’s high performance computing cluster COSMOS through the PReSTO package. General instructions for accessing the PReSTO package and a list of installed software are available here . Specific instructions for running CryoSPARC on COSMOS and sharing projects between users can be found here.