Organisers: Rajmund Mokso, Kajsa Sigfridsson Clauss and Thomas Ursby

Note: This session will be at Stadshallen!

Program:

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8:30 – 9:10 Thomas Boesen, Aarhus University, Denmark
“Structural analysis of membrane associated proteins”
9:10 – 9:30 Gregers Rom Andersen, Aarhus University, Denmark
“Structural basis for a novel immune deficiency”
9:30 – 10:00 Kay Diederichs, University of Konstanz, Germany
“A handle on nonisomorphism: from multi-crystal datasets to serial crystallography”
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10:00 – 10:20 Coffee break
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10:20 – 11:00 David Drew, Stockholm University, Sweden
“Establishing the molecular mechanisms of solute-carrier (SLC) transporters: from snapshots to movies”
11:00 – 11:20 Jennifer Roche, Lund University, Sweden
“AQP2 interaction with lysosomal sorting protein LIP5”
11:20 – 11:50 Zoë Fisher, ESS, Lund, Sweden
“Neutron crystallographic studies of cancer-related human carbonic anhydrase IX reveal details of water displacement and hydrogen bonding in inhibitor binding”
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11:50 – 13:10 Lunch at Grand Hotel – Note: about 10 minutes walk!
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13:10 – 13:50 Anders Larsson, Uppsala University, Sweden
“Microdynamic studies using synchrotron radiation in acute respiratory failure”
13:50 – 14:10 Lars Dahlin, Lund University, Sweden
“X-ray phase contrast zoom tomography of biopsies from human peripheral nerves”
14:10 – 14:30 Sophie Le Cann, Lund University, Sweden
“Mechanical testing using neutron and X-ray tomography to characterize bone-implant interface”
14:30 – 14:50 Patrik Ahvenainen, University of Helsinki, Finland
“Combined in-house X-ray scattering and X-ray microtomography of plant structures”
14:50 – 15:10 Saskia Bucciarelli, Copenhagen University, Denmark
“Amyloid-like protein fibrillation – a highly dynamic and complex process”
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15:10 – 15:40 Coffee break
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15:40 – 16:20 Graham N George, University of Saskatchewan, Canada
“Shedding light on metals in complex systems using X-ray spectroscopy”
16:20 – 16:40 Gustav Berggren, Uppsala University
“Exploring hydrogenases – Using a combination of chemical and biological tools”
16:40 – 17:00 Robert Schnell, Karolinska Institutet, Sweden
“Fatty acid biosynthesis pathway as target for antibiotic development: structural and biochemical characterization of FabG inhibitors”
17:00 – 17:20 Rohit Kumar, Lund University, Sweden
“Galectin-3: Studying molecular recognition in search of inhibitors exhibiting high affinity and selectivity”

 

Session description

The session will give opportunity to present life science applications of protein crystallography, X-ray spectroscopy, small and wide angle scattering, and imaging.

With macromolecular crystallography, the structure of biological molecules can be determined at an atomic level giving insight into the molecular function. The MAX IV beamline “BioMAX” is a microfocus beamline with highly parallel beam making it capable of handling both microcrystals and the largest molecular complexes. The future MicroMAX beamline will open new possibilities in these areas by taking advantage of the latest method developments combined with a micron-sized beam.

X-ray spectroscopy (XAS and XES) provides a detailed element specific picture of the local atomic structure and the electronic states of metal centers in biology. Samples can be in any physical state, which enables studies of enzymatic reaction intermediates also under in situ condition. The Balder beamline will be particular suitable for in situ bio- XAS and XES with focus on sulfur and heavier elements. At the FemtoMAX beamline, ultrafast process can be time-resolved to gain insight into chemical and biological functional dynamics.

Small angle X-ray scattering of biomolecules in solution allows us to study dynamics and model macromolecular systems, for example proteins. The utility of SAXS increases many fold when it is combined with other techniques, from spectroscopy to crystallography and electron microscopy. The CoSAXS beamline at MAX IV will have the capacity to perform high throughput, as well as millisecond time resolution, and rapid on-line HPLC bioSAXS experiments in the generous q-range of 0.0006Å-1 – 6Å-1.

Imaging of biological tissue at the synchrotron beamline in scanning and full-field mode covers the spatial resolution range from several tens of nanometers up to tens of micrometers. This matches the scientific work on intracellular organization up to whole organ studies in small animals. Nano-scale imaging in mainly diffraction contrast will be performed at the NanoMAX and SoftiMAX beamlines, while micrometer-scale full-field imaging shall be implemented at the DanMAX and MedMAX beamlines.

MAX IV offers life science a synchrotron tool box to understand the processes of life from atomic to organism scale. However, the strength to solve the future challenges lays in the combination of techniques using X-rays, neutrons and other lab-based methods.