Beamline Attributes

  • Sample changer (for cryo-data collection)
  • HCLab humidifier (please contact staff in advance)
  • HVE (please contact staff in advance)
  • Beam Size
  • 20 x 5 μm^2 (focused) or 50 x 50 μm^2 (defocused)
  • Beam size at sample defined by a circular aperture (5, 10, 20 or 50 μm diameter)
  • Energy Range6-19.5 keV
    Maximum resolution0.8 Å @ 19.5 keV
    Typical exposure time10 ms / frame

    New features in 2019-2020

    • Higher robot dewar capacity: 29 UniPucks and 464 samples.
    • Minimum sample to detector distance: 146 mm; the resolution at the edge of the detector is 1.1 Å at a wavelength of 0.9 Å.
    • Changes of energy enabled in MXCuBE3. Sample characterization can be enabled upon advanced request.
    • Upgrade of MD3 diffractometer: Faster rastering for fixed target SX experiments.
    • Remote experiment program started in development mode. Please contact staff if you are interested in collecting data remotely.
    • Absorption edge scans for optimized SAD and MAD experiments and automated sample location with mesh/grid scans are not available yet, but we hope to make these features available during 2020.

    Getting beamtime at BioMAX

    The first step to gain access to BioMAX is to submit a proposal for beamtime during the calls for proposals period. Please see the MAX IV user guide for more information about the proposal submission and beamtime application procedures.

    Preparing for beamtime

    • When the user office has notified you about approval of your proposal, please use the DUO interface to apply for an access card and perform the safety quiz. These are compulsory requirements to perform onsite experiments at BioMAX. BioMAX staff will also contact you about suitable times to schedule your beamtime. You also need to sign an Agreement for Access to MAX IV Laboratory. Please read more about the applicable policies here.
    • Once you get beamtime, you will also use DUO to define an experiment session and declare the shifts it will comprise, the number of participants in the experiments and the samples used. Samples samples need to be declared two weeks in advance, except non-hazardous samples, which can be declared up to three days before the experiment.
    • Prepare the samples. The sample exchanger at MAX IV supports SPINE mounts of standard length (cap + pin + loop = 22 mm) mounted in Universal Pucks (UniPucks) only. There are UniPucks available at the beamline in case you do not have any. Please contact staff if you need to borrow the UniPucks. The sample transfer can be done onsite.
    • If you do not bring cryocooled samples need to do extensive sample preparation on-site (beyond cryo-cooling or quick soaks) you will need to arrange in advance training to use the Biolab.
    • After a session has been defined, use ISPyB to arrange sample shipment. This step is compulsory for automated sample mounting with the sample exchanger, even if you are planning to transport the dewar yourself. If you will use the robot, upload a sample information list to ISPyB as described in the documentation.

    Carrying out the experiment

    Note: The starting time for morning experiments is 09:00 (CET) and, for afternoon experiments, 17:30. This differs from the scheduled time in DUO to allow for beamline setup.

    MXCuBE3 is the standard software to collect data at BioMAX. As soon as the data are collected, the software launches several different pipelines to process the data. The results can be accessed any time via ISPyB. We also provide computing facilities for manual reprocessing of the data. Please remember to acknowledge in your publications any tools used at BioMAX to collect and process the data.

    All academic users can use all the software installed on MAX IV computers to process data. However, we may not have appropriate licences for industrial users. If in doubt, please consult with staff.

    Data transfer

    Currently, users belonging to an institution with a valid licence can use Globus for data transfer. We also provide an sftp server for remote data transfer. We do not support the use of external disks as there is often not enough time to copy all the data during the beamtime.

    After the experiment

    Please use the DUO interface to provide feedback about your experiment and report any publications arising from it. Submission of an experimental report is very important for renewal of proposals.

    Remote access tools

    Users with an active proposal and a DUO account can connect to MAX IV from external locations to prepare experiments and reprocess the data. Remote connections are established via VPN. Once connected, the users can log in to a remote desktop with ThinLinc.

    For information about remote experiments, please follow this link.