Beamline Attributes

Equipment
  • Sample changer (for cryo-data collection)
  • HCLab humidifier (please contact staff in advance)
  • HVE (please contact staff in advance)
  • Beam Size
  • 20 x 5 μm^2 (focused) or 50 x 50 μm^2 (defocused)
  • Beam size at sample defined by a circular aperture (5, 10, 20 or 50 μm diameter)
  • Energy Range6-19.5 keV
    Maximum resolution0.8 Å @ 19.5 keV
    Typical exposure time10 ms / frame

    New features in 2019-2020

    During this run we have implemented fully automated changes of energy enabled in MXCuBE3, energy and XRF scans. Automated sample location with mesh/grid scans is not available yet, but we hope to complete implementation before the end of 2020. Remote access is available to general users for most experiment; see Remote experiments at BioMAX.

    For a date-stamped list of short entries of new features and routines, see What’s new @ BioMAX.

    Getting beamtime at BioMAX

    The first step to gain access to BioMAX is to submit a proposal for beamtime during the calls for proposals period. Please see the MAX IV user guide for more information about the proposal submission and beamtime application procedures.

    Preparing for beamtime

    • When the user office has notified you about approval of your proposal, BioMAX staff will contact you about suitable times to schedule your beamtime. You will need to sign an Agreement for Access to MAX IV Laboratory. Please read more about the applicable policies here.
    • Once you get beamtime, please use the DUO interface to apply for an access card (unless the experiment is remote) and perform the safety quiz. You also use DUO to define an experiment session and declare the shifts it will comprise, the number of participants in the experiments and the samples used. Samples samples need to be declared two weeks in advance, except non-hazardous samples, which can be declared up to three days before the experiment.
    • Prepare the samples. The sample exchanger at MAX IV supports SPINE mounts of standard length (cap + pin + loop = 22 mm) mounted in Universal Pucks (UniPucks) only. It is critical that the samples are the correct length, as longer samples can cause the sample changer to collide with the diffractometer. There are UniPucks available at the beamline in case you do not have any. Please contact staff if you need to borrow the UniPucks. The sample transfer can be done onsite.
    • If you do not bring cryocooled samples need to do extensive sample preparation on-site (beyond cryo-cooling or quick soaks) you will need to arrange in advance training to use the Biolab.
    • After a session has been defined, use ISPyB to arrange sample shipment. This step is compulsory for automated sample mounting with the sample exchanger, even if you are planning to transport the dewar yourself. If you ship the samples, please enclose the labels generated by ISPyB, together with the waybill for the return shipment and, if relevant, the customs declaration.

    Carrying out the experiment

    Note: The starting time for morning experiments is 09:00 (CET) and, for afternoon experiments, 17:30. This differs from the scheduled time in DUO to allow for beamline setup.

    MXCuBE3 is the standard software to collect data at BioMAX. As soon as the data are collected, the software launches several different pipelines to process the data. The results can be accessed any time via ISPyB. We also provide computing facilities for manual reprocessing of the data. Please remember to acknowledge in your publications any tools used at BioMAX to collect and process the data.

    All academic users can use all the software installed on MAX IV computers to process data. However, we may not have appropriate licences for industrial users. If in doubt, please consult with staff.

    Data transfer

    Currently, users belonging to an institution with a valid license can use Globus for data transfer. We also provide an sftp server for remote data transfer. We do not support the use of external disks as there is often not enough time to copy all the data during the beamtime.

    After the experiment

    Please use the DUO interface to provide feedback about your experiment and report any publications arising from it. Submission of an experimental report is very important for renewal of proposals.