BioMAX is the first X-ray macromolecular crystallography beamline of MAX IV Laboratory, which begins its user operations in 2017. It is a state-of-the-art resource accommodating multiple cutting edge experimental possibilities. The design goal for BioMAX was to create a stable and reliable beamline that is user friendly. The beamline experiment set-up is highly automated, in terms of both sample handling hardware and data analysis, including feedback on the data collection.
The X-ray beam focus is 20 x 5 μm2 at the sample position with a photon flux of 2 x 1013 ph/s at 500 mA ring current. The operational energy range of the beamline is 5–25 keV. Alternatively, using aperture overfilling it is possible to obtain a stable 5 x 5 μm2 beam at the sample position. Due to its extensive energy tunability, BioMAX is an ideal source for de novo phasing using the anomalous signal of heavy elements. Beam defocusing creates a practically parallel beam, which can be used to resolve extremely large unit cells > 1000 Å at high resolution. Due to its small beam cross-section and optional parallel beam, BioMAX is an optimal experimental set-up for X-ray crystallography using microcrystals and ultra large unit cells.
|Techniques||MX, MAD, SAD, SSAD, Atomic resolution data collection, Large sample ensemble screening, In situ crystal diffraction|
|Beam Size||20 x 5 μm2|
|Energy Range||5-25 keV|
|Samples||Single crystal (1 - 100 μm)|
First Serial Crystallography Experiments performed at BioMAX
“BioMAX serial crystallography setup using a High Viscosity Extrusion (HVE) injector specially designed for the BioMAX endstation by Bruce Doak of the Max Planck Institute for Medical Research, Heidelberg, and fabricated at that institute.” BioMAX has successfully performed the first serial crystallography experiments at the beamline. This new method is performed at room temperature