BioMAX is the first X-ray macromolecular crystallography beamline of MAX IV Laboratory, which is in user operations since 2017. It is a state-of-the-art resource accommodating multiple cutting edge experimental possibilities. The design goal for BioMAX was to create a stable and reliable beamline that is user friendly. The beamline experiment set-up is highly automated, in terms of both sample handling hardware and data analysis, including feedback on the data collection.
The X-ray beam focus is 20 x 5 μm2 at the sample position with a photon flux of 2 x 1013 ph/s at 500 mA ring current. The operational energy range of the beamline is 5–25 keV. Alternatively, using aperture overfilling it is possible to obtain a stable 5 x 5 μm2 beam at the sample position. Due to its extensive energy tunability, BioMAX is an ideal source for de novo phasing using the anomalous signal of heavy elements. Beam defocusing creates a practically parallel beam, which can be used to resolve extremely large unit cells > 1000 Å at high resolution. Due to its small beam cross-section and optional parallel beam, BioMAX is an optimal experimental set-up for X-ray crystallography using microcrystals and ultra large unit cells. Initial synchrotron based serial crystallography (SSX) experiments using an High-Viscosity-Extrusion injector designed and built by Bruce Doak (MPI-Heidelberg) have been carried out.
|Available for||Technique description|
|General Users||Data collection at a fixed energy between 6 and 19.5 keV, detector distance between 220 and 800 mm, with apertures of 5, 10, 20 microns (beam size 20x5microns) or 10, 20 or 50 microns (beam size 50 microns)|
|General Users||Automated sample mounting and dismounting from UniPucks|
|General Users||Sample temperature 100 K; room temperature with or without humidity control available for manual mounting only|
|General Users||Non-optimized SAD experiments (above absorption edge)|
|General Users||Automated data integration, scaling and merging. Offline remote access for manual data processsing.|
|General Users||SX experiments using HVE-injector (High viscosity extrusion injector)|
A successful call for proposals
The spring call for proposals for eight beamlines at MAX IV closed 19 March with a total of 228 proposals from 28 countries. The number of applications is on par with proposals received when the former MAX-lab peaked, which shows that the demand from users is high as well as is their confidence in getting excellent tools