BioMAX is the first X-ray macromolecular crystallography beamline of MAX IV Laboratory, which begins its user operations in 2017. It is a state-of-the-art resource accommodating multiple cutting edge experimental possibilities. The design goal for BioMAX was to create a stable and reliable beamline that is user friendly. The beamline experiment set-up is highly automated, in terms of both sample handling hardware and data analysis, including feedback on the data collection.
The X-ray beam focus is 20 x 5 μm2 at the sample position with a photon flux of 2 x 1013 ph/s at 500 mA ring current. The operational energy range of the beamline is 5–25 keV. Alternatively, using aperture overfilling it is possible to obtain a stable 5 x 5 μm2 beam at the sample position. Due to its extensive energy tunability, BioMAX is an ideal source for de novo phasing using the anomalous signal of heavy elements. Beam defocusing creates a practically parallel beam, which can be used to resolve extremely large unit cells > 1000 Å at high resolution. Due to its small beam cross-section and optional parallel beam, BioMAX is an optimal experimental set-up for X-ray crystallography using microcrystals and ultra large unit cells.
|Techniques||MX, MAD, SAD, SSAD, Atomic resolution data collection, Large sample ensemble screening, In situ crystal diffraction|
|Beam Size||20 x 5 μm2|
|Energy Range||5-25 keV|
|Samples||Single crystal (1 - 100 μm)|
Sample changer for BioMAX in successful user operation
The macromolecular crystallography beamline BioMAX have for the first time been running their sample changer on a regular basis. The robot has been changing 320 samples for four user groups within its first two weeks of operation. At a macromolecular crystallography beamline, the samples are biological molecules. One example is the proteins which are the