BioMAX is the first X-ray macromolecular crystallography beamline of MAX IV Laboratory, which begins its user operations in 2017. It is a state-of-the-art resource accommodating multiple cutting edge experimental possibilities. The design goal for BioMAX was to create a stable and reliable beamline that is user friendly. The beamline experiment set-up is highly automated, in terms of both sample handling hardware and data analysis, including feedback on the data collection.
The X-ray beam focus is 20 x 5 μm2 at the sample position with a photon flux of 2 x 1013 ph/s at 500 mA ring current. The operational energy range of the beamline is 5–25 keV. Alternatively, using aperture overfilling it is possible to obtain a stable 5 x 5 μm2 beam at the sample position. Due to its extensive energy tunability, BioMAX is an ideal source for de novo phasing using the anomalous signal of heavy elements. Beam defocusing creates a practically parallel beam, which can be used to resolve extremely large unit cells > 1000 Å at high resolution. Due to its small beam cross-section and optional parallel beam, BioMAX is an optimal experimental set-up for X-ray crystallography using microcrystals and ultra large unit cells.
|Techniques||MX, MAD, SAD, SSAD, Atomic resolution data collection, Large sample ensemble screening, In situ crystal diffraction|
|Beam Size||20 x 5 μm2|
|Energy Range||5-25 keV|
|Samples||Single crystal (1 - 100 μm)|
Great experience at BioMAX
On June 30, 2017 Professor Jette Sandholm Kastrup, University of Copenhagen were granted two shifts of beamtime at BioMAX by the Program Advisory Committee (PAC) and the MAX IV Laboratory Management for the project “Molecular recognition of agonists, antagonists and positive allosteric modulators at ionotropic glutamate receptors”. The ionotropic glutamate receptors (iGluRs) are highly abundant