BioMAX is the first X-ray macromolecular crystallography beamline of MAX IV Laboratory, which is in user operations since 2017. It is a state-of-the-art resource accommodating multiple cutting edge experimental possibilities. The design goal for BioMAX was to create a stable and reliable beamline that is user friendly. The beamline experiment set-up is highly automated, in terms of both sample handling hardware and data analysis, including feedback on the data collection.

The X-ray beam focus is 20 x 5 μm2 at the sample position with a photon flux of 2 x 1013 ph/s at 500 mA ring current. The operational energy range of the beamline is 5–25 keV. Alternatively, using aperture overfilling it is possible to obtain a stable 5 x 5 μm2 beam at the sample position. Due to its extensive energy tunability, BioMAX is an ideal source for de novo phasing using the anomalous signal of heavy elements. Beam defocusing creates a practically parallel beam, which can be used to resolve extremely large unit cells > 1000 Å at high resolution. Due to its small beam cross-section and optional parallel beam, BioMAX is an optimal experimental set-up for X-ray crystallography using microcrystals and ultra large unit cells. Initial synchrotron based serial crystallography (SSX) experiments using an High-Viscosity-Extrusion injector designed and built by Bruce Doak (MPI-Heidelberg) have been carried out.

Techniques

Available forTechnique description
General UsersData collection at a fixed energy between 6 and 19.5 keV, detector distance between 126 and 900 mm, with apertures of 5, 10, 20 microns (beam size 20x5microns) or 10, 20 or 50 microns (beam size 50 microns)
General UsersAutomated sample mounting and dismounting from UniPucks, 29 puck positions in dewar
General UsersSample temperature 100 K; room temperature with or without humidity control available for manual mounting only
General UsersNon-optimized SAD experiments (above absorption edge)
General UsersAutomated data integration, scaling and merging. Offline remote access for manual data processsing.
General UsersSX experiments using HVE-injector (High viscosity extrusion injector), fixed target scan using the MD3

2019-11-02

First remote operation at BioMAX

At October 31, 2019, the BioMAX team performed together with AstraZeneca the first 8-hour remote operation experiment. The researchers from AstraZeneca in Mölndal connected successfully to the BioMAX beamline remotely (left picture) and collected data from many samples during their 8-hour beamtime. Ana Gonzalez from the BioMAX team observed together with Helena Käck from AstraZeneca